TCEP-HCl锛圖TT鍗囩礆閭勫師鍔戯級鐢熺墿鍒嗗瓙
TECP-HCl 鍩烘湰鎻忚堪锛�
1锛� CAS NO锛�51805-45-9
2锛� 鑻辨枃鍚岀京鍚嶏細TCEP-HCl; Tris (2-Carboxyethyl) phosphine Hydrochloride;
3锛� 涓枃鍚岀京鍚嶏細涓�(2-缇板熀涔欏熀)纾烽菇閰搁菇锛涗笁(2-缇т箼鍩�)鑶﹂菇閰搁菇锛�
4锛� 鍒嗗瓙寮忥細C9H15O6P 路 HCl
5锛� 鍒嗗瓙閲忥細286.65 g/mol
6锛� 澶栬锛氱櫧鑹叉櫠楂�
7锛� 绱斿害锛氣墺99%8锛� 鐔旈粸锛�170-180鈩�9锛� 婧惰В鎬э細婧朵簬姘达紙50mg/ml锛�
TCEP Hydrochloride锛圱ECP-HCl锛�锛�
鑻辨枃鍚峊ris(2-carboxyethyl)phosphine hydrochloride
涓枃鍚嶄笁(2-缇т箼鍩�)鑶﹂菇閰搁菇
CAS锛�51805-45-9
涓€绋笉鍚~閱囦笖鐒¤嚟鐨勫挤閭勫師鍔�锛屽唬娉涚敤浣滃悇绋鑲藉拰铔嬬櫧涓殑浜岀~閸甸倓鍘�銆傛按婧舵€у挤锛屽湪鍫挎€у拰閰告€ф憾娑蹭腑閮借兘绌�(w臎n)瀹氫繚瀛樸€傞倓鍙敤浣滆ū澶氶噸閲戝爆闆㈠瓙濡俍n(II)銆丆d(II)銆� Pb(II)鍜孨i(II)鐨勮灟鍚堝姂銆�
TECP-HCl鐨勫劒(y艒u)鍕紙鑸嘊ME,DTT鐩告瘮杓冿級锛�
鑸囧叾浠栫殑閭勫師鍔戝DTT锛屛�-ME锛圔ME锛夌浉姣�锛孴ECP-HCl鍏锋湁瑷卞鍎�(y艒u)榛烇細
1锛� 鐒¤嚟锛氫笉鍍廌TT鎴朆ME锛岀劇鐣板懗锛屽彲鍦ㄥ伐浣滆嚭涓婇€�(j矛n)琛岃泲鐧芥垨澶氳偨閭勫師瀵﹂锛屽壍(chu脿ng)閫犳洿鍋ュ悍鐨勫椹楃挵(hu谩n)澧�锛�
2锛� 寮风壒鐣版€э細瀵唬pH鑼冨湇鍏�(n猫i)锛岄伕鎿囨€х殑锛屽畬鍏ㄤ笖鍧囧嫽鐨勯倓鍘焭ui绌�(w臎n)瀹氱殑姘存憾鎬т簩纭嵉锛�
3锛� 浣跨敤绨′究锛氫簬瀹ゆ韩锛宲H 5鐨勬浠朵笅锛�5min鍗冲彲鏈夋晥閭勫師锛�
4锛� 绌�(w臎n)瀹氭€ч珮锛歍ECP-HCl鑷韩绲�(ji茅)妲�(g貌u)鐨勫収(n猫i)鍦ㄧ┅(w臎n)瀹氭€у拰灏嶇┖姘f哀鍖栫殑鎶垫姉鎬�锛屼娇寰楄檿鐞嗗拰淇濆瓨鐨勯亷绋嬩腑涓嶉渶鍋氫换浣曢爯(y霉)闃叉帾鏂�銆傝垏铔嬬櫧涓殑鍏朵粬瀹樿兘鍦�(tu谩n)涓嶆渻鐧�(f膩)鐢熷弽鎳�(y墨ng)銆�
5锛� 鏁堢巼楂橈細灏嶄簬澶ч儴鍒嗘噳(y墨ng)鐢�锛�5-50mM TECP-HCl浜庡婧浠朵綔鐢ㄥ咕鍒嗛悩锛屽嵆鍙彁渚涘厖瓒崇殑鎽╃埦鍔涗締閭勫師浜岀~閸点€�
6锛� 鍏煎鎬у唬锛氬洜涓嶅惈纭唶锛屽湪鍩�(zh铆)琛岃ū澶氶Μ渚嗛叞浜炶兒妯�(bi膩o)瑷樻垨浜よ伅(li谩n)瑭﹀姂鐨勫弽鎳�(y墨ng)鍓嶅嬁闇€鎻愬墠鍘婚櫎TECP-HCl銆傚ぇ澶氭暩(sh霉)姊濅欢涓�锛岋紲10-20mM TECP-HCl鍏煎浜庨Μ渚嗛叞浜炶兒鍖栧(xu茅)鍙嶆噳(y墨ng)銆�
1锛� TCEP-HCl鏄撴憾浜庡咕涔庢墍鏈塸H鐨勬按婧舵€х珐娌栨恫锛涢€欎竴鐗规€у厑瑷卞椹楀墠闁嬪婧�(zh菙n)鍌欏伐浣滄恫鍜�10脳鍎插瓨娑�銆�
2锛� TCEP-HCl鍦ㄧ7閰搁菇绶╂矕娑蹭笉鏄緢绌�(w臎n)瀹�锛岀壒鍒ュ湪涓€ф垨鍫挎€H銆傚洜姝�锛岃嫢蹇呴爤浣跨敤婧朵簬PBS鐨凾CEP锛屽缓璀颁娇鐢ㄥ墠鎵嶉枊濮嬮厤鍒跺伐浣滄恫銆�
3锛� TCEP-HCl鍙兘鐢ㄤ綔DTT鍜孊ME鐨勬浛浠g墿锛岀敤浜嶴DS-PAGE瀵﹂鐢ㄧ殑妯e搧涓婃ǎ绶╂矕娑�锛涗娇鐢ㄧ祩婵冨害鐐�50mM銆�
4锛� 鐢变簬TCEP-HCl鍦ㄦ憾娑蹭腑甯舵闆昏嵎锛屽洜姝や笉鑳借垏绛夐浕榛炶仛鐒︽噳(y墨ng)鐢ㄥ吋瀹�銆�
姝¤繋鏂拌€佸鎴朵締闆伙紝鎴戝€戝皣浠ョ啽鎯呯殑鏈嶅嫏(w霉)锛屽劒(y艒u)璩�(zh矛)鐨勭敘(ch菐n)鍝佺瑾犵偤鎮ㄦ湇鍕�(w霉)锛�
璩艰卜娴佺▼锛�
1銆�闆昏┍鑱�(li谩n)绯婚姺鍞儴浜哄摗锛岀⒑瑾�(r猫n)鐢�(ch菐n)鍝佸悕绋憋紝鏁�(sh霉)閲�锛屽児鏍�锛屽寘瑁濈瓑
2銆�纰鸿獚(r猫n)鐒¤鍚庡尟娆捐嚦鎴戝叕鍙歌超鎴讹紝骞剁櫦(f膩)閫佽┏绱�(x矛)鏀朵欢淇℃伅
3銆�璨″嫏(w霉)鍒版鍚庝笅鍠畨鎺掞紝2鍊嬪伐浣滄棩鍏�(n猫i)鐧�(f膩)璨�
4銆�瀹㈡埗纰鸿獚(r猫n)鏀惰波
娉ㄦ槑锛氬闇€瑕佺壒娈婂寘瑁�锛屾寚瀹氬揩閬炵殑闇€瑕佹彁鍓嶈垏閵峰敭閮ㄤ汉鍝℃簼閫�
婀栧寳姝h垐婧愮簿绱�(x矛)鍖栧伐鏈夐檺鍏徃锛屽墠韬疁鍩庢鍏冨寲宸ユ湁闄愬叕鍙革紝鎴愮珛浜庝簩闆堕浂涓€骞村叓鏈�锛屽叕鍙镐互鐮旂┒銆佺敓鐢�(ch菐n)銆侀姺鍞偤涓€楂�锛屽骞翠締鑷村姏浜庣爺鐧�(f膩)鍜岀敓鐢�(ch菐n)閱�(y墨)钘ュ師鏂欒棩鍙婇啱(y墨)钘ヤ腑闁撻珨銆傛槸涓€瀹朵互鐮旂櫦(f膩)鐐哄熀绀�(ch菙)銆佷互鍙寔绾�(x霉)鐧�(f膩)灞曠偤灏�(d菐o)鍚戠殑鍦嬪閲嶉粸楂樻柊鎶€琛�(sh霉)浼佹キ(y猫)銆�
鍏徃寤犲崁(q奴)鍗犲湴闈㈢⿳22000骞虫柟绫�锛屾搧鏈夌従(xi脿n)浠e寲鐨勬綌鍑堝粻鎴垮拰鍏堥€�(j矛n)鐨勭敓鐢�(ch菐n)瑷�(sh猫)鍌�锛屾妧琛�(sh霉)鍔涢噺鍗佸垎闆勫帤锛岀敓鐢�(ch菐n)閬庣▼鍤�(y谩n)鏍奸伒寰瀛�(xu茅)瑕�(gu墨)鑼冪殑鎿嶄綔娴佺▼銆傚悓鏅傚叕鍙告搧鏈夊厛閫�(j矛n)瀹屽倷鐨勫垎鏋愭娓剙鍣�锛屽搧璩�(zh矛)淇濊瓑閮ㄥ叏绋嬬洠(ji膩n)鎺у緸鍘熸枡閲囪臣鍏ュ粻鍒扮祩鐢�(ch菐n)鍝佸嚭搴殑鎵€鏈夌挵(hu谩n)绡€(ji茅)锛屽畬鍠勭殑璩�(zh矛)閲忔娓拰璩�(zh矛)閲忎繚璀夐珨绯荤⒑淇濇垜鍊戝悜甯傚牬鎻愪緵绌�(w臎n)瀹氱殑楂樺搧璩�(zh矛)鐢�(ch菐n)鍝併€�